m PA-C AGAR

Technical Data #1631a / 98.06.10

m-PA-C Agar is a selective solid medium for detection and enumeration of Pseudomonas aeruginosa from water. Various carbohydrates provide energy sources required. Phenol red is the pH indicator.

 

FORMULA
in grams per liter purified filtered water

 

Yeast Extract

2,0
L-Lysine HCl

5,0
Lactose

1,25
Sodium Chloride

5,0
Sodium Thiosulfate

5,0
Xylose

1,25
Sucrose

1,25
Magnesium Sulfate

1,5
Ferric Ammonium Citrate

0,8
Phenol Red

0,08
Agar

13,5

pH 7,2 +/- 0,2 at 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25º C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 36,5 g of dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. DO NOT AUTOCLAVE. Cool to 45-50º C. Add 0,008 g of sterile solution of Kanamycin and 0,037 g of sterile solution of Nalidixic Acid. Mix gently and dispense into sterile Petri dishes.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Place the membrane filter used to collect the water sample, on the surface of the agar. Avoid air bubbles between surface of agar and membrane filter.
  2. Incubate at 42º C for 24 hours.


QUALITY CONTROL
Results after 24 hrs at 42ºC

 

Organisms

ATCC

Growth
Pseudomonas aeruginosa

27853

+
Escherichia coli

25922

- or partial

LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Brodsky, M.H., B.W. Ciebin 1978 Improved medium for recovery and enumeration of Pseudomonas aeruginosa from water using membrane filters. Appl. Environ. Microbiol. 36:26
  2. Levin, M.A., V.J. Cabelli 1972 Membrane filter technique for enumeration of Pseudomonas aeruginosa Appl. Microbiol. 24:864.
  3. Standard Methods for Examination of Water and Wastewater 1989 17th ed. APHA Washington D.C.


CATALOGUE NUMBERS

 

Dehydrated media 500 g QB-39-3007
     
Prepared media    
Plates, 100x15 mm 10/pkg 1631