MYCOBIOTIC GENTAMYCIN AGAR

Technical Data #1922a / 98.06.15

Mycobiotic Gentamycin Agar is a solid selective medium for isolation of pathogenic fungi from materials contaminated with other fungi and bacteria.

 

FORMULA
in grams per liter purified filtered water

 

Soy Peptone

10,0
Dextrose

10,0
Cycloheximide

0,4
Chloramphenicol

0,05
Agar

15,5
Additionnal antibiotic

 
Gentamycin

0,0075

pH 6,9 +/- 0,2 at 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25º C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.


DIRECTIONS
Suspend 36 g of dehydrated media in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121º C for 15 minutes. Cool to 45-50º C. Add 10 ml of Gentamycin Supplement (8698). Mix gently and dispense into sterile Petri dishes.


PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

  1. Directly, inoculate plate as soon as possible with specimen and streak for isolation. For cutaneous specimens, press specimen gently on the surface of the agar.
  2. Tape the plates and incubate at 25º C for 4-6 weeks.


QUALITY CONTROL
Results after 7 days at 25ºC

 

Organisms

ATCC

Growth
Trichophyton mentagrophytes

9533

+
Candida albicans

10231

+
Aspergillus niger

16404

- or partial
Escherichia coli

25922

- or partial

LIMITATIONS OF METHOD
As some fungi may be inhibited by the antibiotics in this medium, a general purpose medium such as Sabouraud Dextrose Agar should be inoculated simultaneously. This medium is only a part of the identification. Other tests may be required.


REFERENCES

  1. Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.
  2. Mac Faddin, Jean F., 1985 Media for Isolation-Cultivation-Identification-Maintenance of Medical Bacteria. Vol.1. Baltimore, MD.: Williams & Wilkins.


CATALOGUE NUMBERS

 

Dehydrated media 500 g QB-39-3020
     
Gentamycin Supplement: 10 ml 10/pkg 8698
     
Prepared media    
Plates, 100x15 mm 10/pkg 1922