B.H.I. (BRAIN HEART INFUSION) ANAEROBE BROTH

B.H.I. ANAEROBES BROTH WITH BLOOD

Technical Data #2458a / 98.06.25

B.H.I. anaerobes broth with blood is used for cultivation and maintenance of anaerobic microorganisms. Yeast extract enhances growth of many bacteria and hemin-vitamin K1 improve anaerobes growth. The addition of L-cysteine reduces oxygen tension for anaerobes growth.

 

FORMULA
in grams per liter purified filtered water

Brain Heart Infusion/Meat Peptone

12,0

Casein Peptone

15,0

Yeast Extract

5,0

Dextrose

2,0

Sodium Chloride

5,5

Dipotassium Phosphate

2,5

Cystein HCl

0,5

Supplements

 

Hemin

0,005

Vitamin K

0,010

Defibrinated Sheep Blood

50 ml

 

pH 7,4 +/- 0,2 at 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for IN VITRO diagnostic use only.


STORAGE
Store prepared media at 2-8º C protected from direct light. Medium must not be used if there are signs of contamination or if the expiry date has passed.


PROCEDURE
Bring tubes to room temperature before inoculation.

  1. Inoculate specimens as soon as possible. Tighten the caps.
  2. Incubate at 35º C for 24-72 hours and then up to 7 days
  3. Examine tubes daily for visual signs of growth.


QUALITY CONTROL
Expected results after 48 hrs at 35ºC

Organisms

ATCC

Growth

Bacteroides fragilis

25285

+

Bacteroides levii

29147

+

Bacteroides vulgatus

8482

+

Clostridium novyi

7659

+

Clostridium perfringens

13124

+


LIMITATIONS OF METHOD
This is only a part of the identification. Other tests may be required.


REFERENCES

  1. Balows,A., Hausler Jr. W.J., Herrmann, K.L., Isenberg, H.D., Shadomy, H.J., 1991. Manual of Clinical Microbiology 5th ed. American Society for Microbiology Washington, D.C.
  2. Finegold S.M, Baron, E.J. 1986 Bailey and Scott's Diagnostic Microbiology 7th ed. C.V. Mosby.
  3. Mc Faddin, J.F. 1985 Media for isolation-cultivation-identification-maintenance of medical bacteria. Vol. 1 Williams & Wilkins, Baltimore


CATALOGUE NUMBERS

Dehydrated media

500 g

QB-39-0305

 

 

 

Prepared media

 

 

Tubes 16x125 mm, 5 ml

10/pkg

2458

 

 

 

Supplements

 

 

Vitamin K-Hemin, 5ml

10/pkg

8752

 

 

 

Defibrinated Sheep Blood

50 ml

4245