N.Y.C. (NEW YORK CITY) AGAR

Technical Data # 1865a / 2010.08.19

N.Y.C. Agar is an enriched selective medium used for the isolation and cultivation of fastidious bacteria and pathogenics Neisseria sp. Hemolysed horse blood, horse serum, dextrose and yeast dialysate are the enrichments which permit a luxuriant recovery of pathogenic Neisseria sp. Vancomycin, a gram positive cocci inhibitor, is replaced by lincomycin to prevent inhibition of some strains of Neisseria gonorrhoeae. Trimethoprim lactate prevents Proteus sp. Swarming, while Colimycin inhibits gram negative bacilli, amphotericin B suppress yeast growth.

FORMULA
in grams per liter purified filtered water

Casein Peptone	7,5
Meat Peptone	7,5
Corn Starch	1,0
Potassium Phosphate, dibasic	4,0
Potassium Phosphate, monobasic	1,0
Sodium Chloride	5,0
Agar	10,0
Enrichments
Dextrose 50%	10 ml
Yeast Dialysate	25 ml
Horse Serum	120 ml
Hemolysed Horse Blood	75 ml
Antibiotics
L.C.A.T	10 ml

pH 7,4 +/- 0,2 at 25º C

This approximate formula may be adjusted and/or enriched to obtain best results.

PRECAUTIONS
This medium is for IN VITRO diagnostic use only.

STORAGE
Store prepared media at 2-8º C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25º C.

SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.

DIRECTIONS
Suspend 36 g of dehydrated media in 750 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Sterilize at 121º C for 15 minutes. Cool to 45-50º C. Add 10 ml Dextrose 50% (8580), 25 ml of Yeast Dialysate (8643), 50 ml of Horse Serum (4602), 20 ml of Hemolysed Horse Blood (4177) and 10 ml of LCAT (8610). Mix gently and dispense into sterile Petri dishes.

PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

Directly, inoculate plate as soon as possible with specimen and streak for isolation.
Incubate at 35º C with 5% CO2 for 48 hours.

QUALITY CONTROL
Results after 48 hrs at 35ºC w/ CO2

Organisms	ATCC	Growth
Neisseria gonorrheae	43069	+
Neisseria gonorrheae	clinical	+
Neisseria meningitidis	13090	+
Proteus mirabilis	43071	- or partial
Escherichia coli	25922	- or partial
Neisseria sicca	9913	-
Candida albicans	60193	- or partial
Staphylococcus epidermidis	12228	- or partial

LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.

REFERENCES

Faur, Y.C., M.H. Weisburd, M.E. Wilson 1978 The selectivity of vancomycin and lincomycin in NYC medium for the recovery of N. gonorrheae from clinical specimens. Health Lab. Sci. 15:22-27
Granato, P.A., C. Schneible-Smith, L. B. Weiner 1981 Use of New York City Medium for improved recovery of Neisseria gonorrheae from clinical specimens 13:963-968
Lennette, E.H., Ballows, A., Hausler, W.J.Jr., and Shadomy, H.J. Manual of Clinical Microbiology. 4th ed. 1985 Washington D.C.: American society for Microbiology.

CATALOGUE NUMBERS

Dehydrated media	500 g	QB-39-1906

Prepared media
Plates, 100x15 mm	10/pkg	1865
Bi–plaque: Choco/NYC, 100x15 mm	10/pkg	1565
Jembec NYC modified, rectangular	10/pkg	1410

Dextrose 50%	100 ml	8580
Yeast Dialysate	25 ml	8643
Horse Serum	150 ml	4602
Lysed Horse Blood	100 ml	4177
LCAT Supplement, 10 ml	10/pkg	8610