L.B. (LURIA-BERTANI) MILLER 10 gL SODIUM CHLORIDE BROTH

Technical Data # 39-2405a / 2010.08.31

L.B. Broth, Miller medium is used for the growth and maintenance of Escherichia coli strains used in molecular microbiology procedures.

FORMULA
in grams per litre purified filtered water

Enzymatic Digest of Casein......................... 10 g

Yeast Extract ................................................. 5 g

Sodium Chloride .......................................... 10 g

pH 7.3 ± 0.2 at 25̊ C

This approximate formula may be adjusted and/or enriched to obtain best results.

PRECAUTIONS
This medium is for laboratory use only. Irritating to eyes, respiratory system, and skin.

STORAGE
Store prepared media at 2-30° C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers.

SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration).

DIRECTIONS

Suspend 25 g of the medium in 1000 mL of distilled purified water. Heat with frequent agitation and boil for one minute. Sterilize at 121° C for 15 minutes.

QUALITY CONTROL

Results at 35°C after 18 - 24 hours incubation.

Microorganisms ATCC	Response
Bacillus subtilis 9372	growth
Escherichia coli 25922	growth

LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.

REFERENCES

1. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory. Cold Spring Harbor, New York.

2. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring HarborLaboratory, Cold Spring Harbor, New York.

CATALOGUE NUMBER

Deshydrated medium 500 g QB-39-2405