MANNITOL SALT CHAPMAN AGAR

Technical Data # 1858a / 2010.08.30

Chapman formulated Mannitol Salt Agar is used for isolate staphylococcus by inhibiting growth of most other bacteria with a high salt concentration. 

 

FORMULA
in grams per litre purified filtered water

Enzymatic Digest of Casein....................5 g

Enzymatic Digest of Animal Tissue........5 g

Beef extract...........................................1 g

D- Mannitol..........................................10 g                                                    

Sodium Chloride...................................75 g

Phenol Red......................................0.025 g

Agar.....................................................15 g 

 

pH 7.4 ± 0.2 at 25 ̊ C

This approximate formula may be adjusted and/or enriched to obtain best results.

PRECAUTIONS
This medium is for laboratory use only. Irritating to eyes, respiratory system and skin.

STORAGE

Store prepared media at 2-30° C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25° C.

SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.

DIRECTIONS

Suspend 111 g of the medium in 1000 ml of purified filtered water. Heat with frequent agitation and boil for one minute. Autoclave at 121 ̊C for 15 minutes.

PROCEDURE

Inoculate specimen on medium as a primary isolation or inoculate isolated colonies onto medium for differentiation.

Microorganisms        ATCC

Approx. Inoculum (CFU)

Expected Results

Recovery

Reaction

Proteus mirabilis        12453

300 - 1000

Partial to complete inhibition

Colorless

Staphylococcus aureus 6538

10 - 100

Fair to good

Yellow colonies; may have yellow halo around colonies.

Staphylococcus aureus                     25923

10 - 100

Fair to good

Yellow colonies; may have yellow halo around colonies

Staphylococcus

epidermidis              12228

10 - 100

Fair to good

Colorless to pink colonies

Escherichia coli          8739

300 - 1000

Inhibited

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LIMITATIONS OF METHOD

The organisms listed are the minimum that should be used for quality control testing. This medium is only a part of the identification. Other tests may be required. 

REFERENCES

1.     United States Pharmacopeial Convention. 2007. The United States pharmacopeia, 31st  ed., Amended Chapters 61, 62, 111. The United States Pharmacopeial Convention, Rockville, MD.

2.     Directorate for the Quality of Medicines of the Council of Europe (EDQM). 2007. The European Pharmacopoeia, Amended Chapters 2.6.12, 2.6.13, 5.1.4, Council of Europe, 67075 Strasbourg Cedex, France.

3.     Japanese Pharmacopoeia. 2007. Society of Japanese Pharmacopoeia. Amended Chapters 35.1, 35.2, 7. The Minister of Health, Labor, and Welfare.

4.     Chapman, G. H. The significance of sodium chloride in studies of staphylococci. J. bacteriol. 50:201.

5.     Kloos, W. E., and T. L. Bannerman. 1995. Staphylococcus and Micrococcus. In P. R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (eds.). Manual of clinical microbiology, 6th ed. American Society for Microbiology, Washington, D.C.

6.     Hitchins, A. D., T. T. Tran, and J. E. McCarron. 1995. Microbiology methods for cosmetics, p. 23.01-23.12. In Bacteriological analytical manual, 8th ed. AOAC International, Gaithersburg, MD.

CATALOGUE NUMBER

Prepared dehydrated   500g            QB-39-2906

Prepared medium

Petri plate                    10/pkg               1858

Petri plate contact          10/pkg               1403