UREA BROTH

Technical Data # 39-5307a / 2010.08.30

Urea Broth is used for the differentiation of Enterobacteria, particularly Proteus from Salmonella and Shigella, as well as microorganisms of the families of Brucella, Bacillus, Micrococcus,  Mycobacteria and  Proteus.

 

FORMULA
in grams per litre purified filtered water

Urea......................................................20.00 g

Dipotassium Phosphate..........................9.50 g

Monopotassium Phosphate....................9.10 g

Yeast Extract..........................................0.10 g

Phenol Red............................................0.01 g

pH 6.8 ± 0.2 at 25 ̊ C

This approximate formula may be adjusted and/or enriched to obtain best results.


PRECAUTIONS
This medium is for laboratory use only.


STORAGE
Store prepared media at 2-30° C protected from direct light and dehydrated powder, in a dry place, in tightly-sealed containers at 2-25° C.


SIGN OF DETERIORATION
Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration (shrinking, cracking, evaporation or discoloration). Do not use dehydrated media if it is caked.

DIRECTIONS

Suspend 3.87 grams of the medium in 100ml of distilled water without heating. When the powder is dissolved, sterilize by filtration. Dispense  in small sterile tubes  quantities  of 0.5 to 2 ml. Larger volumes can be used but the reactions will be slower. When there is no filter available the medium can be sterilized at 100 -110°C for 10 minutes. If the medium is prepared and inoculated immediately it provides good results without sterilizing.

QUALITY CONTROL

Results were obtained in the performance of the medium from type cultures after incubation at a temperature of 35 ± 2°C and observed  after 24 hours.

 

Microorganisms                ATCC           Growth                          

Urease Escherichia  coli       25922             -                         

Klebsiella pneumoniae        13883             +                            

Salmonella  typhimurium    14028              -                    

Proteus   vulgaris               13315               +                        

LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.


REFERENCES

Rustigian and Stuart.  Proc.  Soc. Exp. Biol. and Med. 47:109, 1941. Stuart,  Van Stratum and Rustigian. J. Bact. 48:437. 1945. McKay, Edwards and Leonar A. J. Clin. Path. 17:479, 1947. Gordon and Mihn. J. Gen. Microbiol., 21:736. 1959. Goldsmith and Latlief. Applied Microbiol., 3:195. 1955.

CATALOGUE NUMBER

Deshydrated medium            500 g                QB-39-5307