UREAPLASMA AND MYCOPLASMA, TRANSPORT MEDIA BROTH

Technical Data # 2281a / 2010.09.03

 

The transport media broth for Ureaplasma and Mycoplasma is used for sampling and transport of Mycoplasma sp. et Ureaplasma sp. The casein and soya peptones enrich with albumin serve to keep these microorganisms lived instead of penicillin, which eliminate mixed normal flora.

 

FORMULA
in grams per litre purified filtered water

 

Casein Peptone                                               17,0

Soya Peptone                                                  3,0

Sodium Chloride                                              5,0

Dextrose                                                        2,5

Dipotassium Phosphate                                     2,5

Enrichment

Bovine albumine, fraction V                               5,0

Antibiotic

 Penicillin                                                       1,0 MM units

 

                               pH 7,3 +/- 0,2 at 25o C

This approximate formula may be adjusted and/or enriched to obtain best results.

PRECAUTIONS
This medium is for laboratory use only.

STORAGE
Store prepared media at 2-8° C protected from direct light. Media should not be used if the expiry date has passed. Prepared media should not be used if there are signs of contamination or deterioration.

PROCEDURE
Prior to inoculate, the prepared media should be brought to room temperature.

1. Directly, inoculate the specimens. Dip swabs and break into culture media and cap appropriateness.

2. Transfer 0,1 ml of transport medium for Mycoplasma and Ureaplasma in an arginine broth (#2991), or in a Ureaplasma broth U9 (#2361).

3. If it’s a delay in inoculation, the transport medium must be refrigerated at 4-8o C for 2 days maximum. The transport media must be congealed at –20o C for a maximum of 7 days. For a long and indefinite delay congealed at –70o C

4. Incubate at 35o C for 24 –72 hours and discharged the medium after 7 days.

5. Examine the broths each day and note the color change.  

QUALITY CONTROL
Results after 48 hrs at 35ºC

     Organisms                     ATCC             Growth         

Ureaplasma urealyticum      27618                  +                   

Mycoplasma hominis           23114                 +                   

Escherichia coli                  25922                  -                   

Staphylococcus aureus       25923                   -                   

Candida albicans               60193                   -                   

 

LIMITATIONS OF METHOD
This medium is only a part of the identification. Other tests may be required.

REFERENCES

1- Kenny, G.E., Manual of Clinical Microbiology 1985  Manual of Clinical Microbiology 4th, ed. Chap. # 36 pp. 407-411, A.S.M.

2- Lefebvre, J., Public Health Laboratory, D.S.C. St. Luc Hospital, Ste-Anne-de-Bellevue                Pesronnal Communications.

3- Clyde, W.A., G.E. Kenny, and J. Schachter, Chlamydial and mycoplasmal infections 1984 Cumitech 19L.A., A.S.M.

4- Shepard, M.C., and C.D. Lanceford 1976 Differential agar medium (A7) for the identification of Ureaplasma urealyticum (human T-strain) in primary cultures of clinical materials. J. Clin. Microbiol. 3 :613-625.

5- Balows, A., and W.J. Hausler, 1981 Diagnostic Procedures for Bacterial, Mycotic and Parasitic Infections, 6th ed.

6- Taylor-Robinson, D., 1983 Recovery  of Mycoplasmas from the genito-urinary tract. Methods in Mycoplasmalogy. Academy Press Inc. New York, Vol II. 19-26.

7- Tully, J.D., D. Taylor-Robinson, D.L. Rose, P.M. Furr, D.A. Hawkins 1983 Evaluation of culture media for recovery of Mycoplasma hominis from the human Urogenital tract. Sexually Transmitted Disease, Vol. 10 4:256-260

CATALOGUE NUMBERS

Prepared medium

Transport for Mycoplasma and Ureaplasma

Tubes 12x75 mm, 2 ml             10/pkg               2281

Tubes 13x100 mm, 2 ml           10/pkg               2989